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How Long Can Frozen Sperm Survive in the Womb After IUI?

Find out how long frozen sperm can survive in the uterus after IUI, factors that affect its lifespan, and what this means for your chances of conception.

Getting Pregnant

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Published on 6 Aug 2025

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By Thomson Team

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After your IUI procedure, you may be wondering how long frozen sperm can remain active inside the womb and whether timing affects your chances of conceiving. Understanding this can make the two‑week wait a little less stressful and help you to know what to expect.

What is frozen sperm?

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Frozen sperm, also known as cryopreserved sperm, is a semen sample that has been carefully collected, tested for safety, and then preserved through freezing. During this process, a protective substance called a cryoprotectant is added before the sperm is stored in liquid nitrogen at -196°C.

This method is commonly used in fertility treatments, including:

  • Donor insemination (using sperm from a donor).

  • Fertility preservation before medical treatments like chemotherapy or cancer treatment.

  • IUI (intrauterine insemination) and IVF (in vitro fertilisation) procedures, sometimes in combination with a donor egg.

  • Cases where timing or distance requires flexibility in sperm use. 

How is frozen sperm used in IUI procedures?

During an IUI, frozen sperm undergoes a few careful processes before it’s placed into the womb:

  • Thawing: 

    • The frozen sperm is carefully thawed at the clinic, usually 30-45 minutes before the procedure.

  • Preparation: 

    • The sample is washed and concentrated to separate healthy, motile sperm from seminal fluid and non‑motile cells, ensuring a sufficient post-thaw sperm count for insemination.

  • IUI procedure: 

    • The prepared sperm is inserted directly into the uterus using a thin catheter, typically 24-36 hours after ovulation is triggered.

Only a small amount (around 0.3-0.5 mL of concentrated sperm) is used during IUI, which is enough to maximise fertilisation potential while minimising discomfort.

How long does frozen sperm survive after IUI?

Once frozen sperm is thawed and placed into the uterus during IUI, it can typically survive for about 12 to 72 hours. However, its fertilisation potential is highest in the first 24 to 48 hours.

This is why timing the insemination closely with ovulation is important, as it ensures the sperm and egg meet at the right moment, giving you the best chance of conception. 

What factors affect frozen sperm survival?

Here are several factors that can influence how well frozen sperm survives and functions after IUI:

FactorImpact on survival
Freezing and thawing techniqueThe method used to freeze and thaw sperm affects its motility and overall viability.
Sperm quality before freezingHigher-quality sperm at the time of freezing generally survive the process better.
Use of cryoprotectantsSpecial protective agents prevent ice crystals from damaging the sperm during freezing.
DNA integrityAny damage to the sperm’s DNA during the freeze-thaw cycle can reduce its fertilising ability.
Storage conditionsConsistent storage in liquid nitrogen keeps sperm viable for many years.

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How safe is sperm freezing?

Sperm freezing is considered very safe and is widely used in fertility care around the world. The process is strictly regulated to protect both recipients and future children.

  • All sperm samples are screened for infections such as HIV and hepatitis before storage.

  • Long‑term studies show no increase in birth defects from using frozen sperm compared to fresh sperm.

  • Sperm can remain viable for decades, with reported pregnancies from sperm stored for more than 20 years.

If you’re considering IUI or other fertility treatments, knowing that frozen sperm is safe and reliable can give you peace of mind.

How to get pregnant with frozen sperm

There are a few ways to conceive using frozen sperm, depending on your fertility plan and conditions, these include:

  • IUI (intrauterine insemination):

    • IUI is the most common approach, where prepared sperm is placed directly into the uterus.

  • IVF (in vitro fertilisation):

    • IVF is often used if IUI is unsuccessful or if there are age‑related or egg quality concerns.

  • ICI (intracervical insemination):

    • A less common method, sometimes done at home, where sperm is placed near the cervix. The key difference is that IUI places sperm directly into the uterus, while ICI deposits it at the cervix to move naturally into the uterus.

Typical steps in the process:

  • Order frozen sperm from a certified sperm bank

  • Track or trigger ovulation to ensure proper timing

  • Thaw, prepare, and insert the sperm (usually via IUI at a clinic)

  • Wait about 14 days before taking a pregnancy test

Working closely with a fertility specialist can help you choose the best method for your fertility condition and improve your chances of success. If you’re considering IUI or IVF with frozen sperm, request an appointment with Thomson Fertility Centre to discuss your options and next steps.

FAQ

How long does frozen sperm stay viable?

Frozen sperm can remain viable indefinitely as long as it is stored at -196°C in liquid nitrogen. In fact, there have been documented cases of successful pregnancies using sperm that had been frozen for over 20 years.

What is the success rate of IUI with frozen donor sperm?

The success rate of IUI with frozen donor sperm depends on factors like age, reproductive health, and timing. On average:

  • 15-20% per cycle if you’re under 35 years old
  • Around 40–50% cumulative success after 3-4 cycles

Success rates are comparable to IUI with fresh sperm when insemination is timed accurately and the uterine lining and ovulation are optimal.

Is it harder to get pregnant with frozen sperm?

Getting pregnant with frozen sperm is not significantly harder than with fresh sperm–as long as the semen sample was good quality before freezing. While sperm motility can drop slightly after thawing, sperm banking facilities only release samples that meet strict standards, usually with motility above 40% before freezing and around 30-40% after thawing.

Does frozen sperm lose quality?

Frozen sperm may lose a some of its quality after thawing, mainly in terms of motility, which can drop by about 30-50%. However, its morphology (shape) and DNA integrity are usually well preserved if the freezing process is done correctly.

Before being used for IUI, sperm is always washed and tested to ensure it meets safe and effective standards. Most sperm banks provide samples with at least 5-10 million motile sperm after thawing, which is more than enough for a successful IUI procedure.

Are babies from frozen sperm healthy?

Yes. Babies conceived using frozen sperm are just as healthy as those conceived with fresh sperm. Research shows no increase in birth defects, developmental issues, or health problems linked to sperm freezing.

Sperm banks and fertility clinics follow strict screening and storage standards to ensure sperm quality and safety. This includes testing for genetic conditions and infectious diseases before freezing, as well as monitoring storage conditions to maintain viability for years–even decades.

Is frozen sperm less effective in IUI compared to fresh sperm?

Not necessarily. While frozen sperm may experience a slight drop in motility after thawing, studies show that IUI success rates are very similar between frozen and fresh sperm when timing and preparation are done correctly.

Your fertility specialists will carefully wash, test, and prepare frozen sperm before insemination to ensure only healthy, motile sperm are used. As a result, frozen sperm remains a highly effective option for IUI, especially when fresh sperm isn’t available or timing flexibility is needed.

The information provided is intended for general guidance only and should not be considered medical advice. For personalised recommendations and tailored advice, please consult a specialist at Thomson Medical. Request an appointment with Thomson Medical today.

For more information, contact us:

Thomson Fertility Centre

Thomson Specialists (Women's Health)

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